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ABclonal Biotechnology tet2
Tet2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tet2/TET2+Rabbit+pAb/pm42587291-127-31-35
Average 94 stars, based on 18 article reviews

tet2 - by Bioz Stars, 2026-10

94/100 stars

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Cell-Signaling:

Article Title: Acetoacetate-GPR43 axis epigenetically silences IL-6/CSF1 to restrict TAMs-driven metastatic lung cancer.
Article Snippet: Protein signals were detected using an ECL reagent (Epizyme, China) and visualized with the Tanon 5200 imaging system (Tanon, China). .. The primary antibodies used included: HIF1α, IKBα, p-IKBα, P65, p-P65, p-JNK, p-c-Jun, LaminA/C, H3K27me3, H3K27ac, H3K18ac, H3K9ac, H3, DNMT1 (Cell Signaling Technology, United States); FFAR2/GPR43, DNMT3A, DNMT3B, MAT2A (Proteintech, United States); TET2 (Abcam, UK); p-STAT3 (ABclonal, China); p-TAK1 (Abmart, China); p44/42 MAPK (Erk1/2), Phospho-p44/42 MAPK (Erk1/2) (T202/Y204) (Selleck, USA); β-Tubulin (Cwbiotech, China); and β-actin (HuaBio, China). ..

Incubation:

Article Title: Tet2 acts in the lateral habenula to regulate social preference in mice.
Article Snippet: .. Frozen sections were incubated with the blocking solution (PBS with 0.2% Triton X-100 and 2% bovine serum albumin) for 1 h. Sections were then incubated with primary antibodies overnight at 4 C. Primary antibodies in this study included Tet2 (1:100, ABclonal, China), NeuN (1:500, Abcam, Britain), GFAP (1:500, Millipore, USA), S100b (1:200, ABclonal, China) and Iba1 (1:300, Fujirebio, Japan). .. On the second day, the sections were incubated with secondary antibodies conjugated with Alexa Fluor 647 (1:800, Life Technologies, USA) or Alexa Fluor 594 (1:300, Jackson ImmunoResearch Laboratories, USA) for 2 h at room temperature.

Article Title: SARS-CoV-2-induced hypomethylation of the ferritin heavy chain (FTH1) gene underlies serum hyperferritinemia in severe COVID-19 patients.
Article Snippet: .. The membrane was washed 3 times with 1X TBST and incubated with a primary antibody against FTH (LS-B11085-200; LS Bio, Seattle, WA, USA; at 1:1000), DNMT1 (5032S; Cell Signaling Technology, Danvers, Massachusetts, United States), DNMT3A (ab232391; Abcam, Waltham, MA, United States), DNMT2B (ab239893; Abcam), G9a (D5R4R; Cell Singling Technology, Danvers, MA, USA; at 1:1000), TET1 (A1506, Abclonal), TET2 (A5682, abclonal), TET3 (A18319, abclonal) or GAPDH (8884; Cell Signaling Technology) at 4 C overnight. .. The primary antibody solution was discarded and membranes werewashed 3 times with 1X TBSTand incubated with a secondary antibody (anti-mouse [Cat. No. 7076; Cell Signaling Technology; at 1:1000] or anti-rabbit antibody [ac97040; Abcam; at 1:5000] in 3% BSA in 1X TBST for 2 h at room temperature.

Article Title: High fat diet-induced- and genetically inherited- obesity differential alters DNA demethylation pathways in the germline of adult male rats.
Article Snippet: Obesity is a multifactorial condition with predominantly genetic and environmental causes and is an emerging risk factor for male infertility/subfertility.. Epigenetic mechanisms are vulnerable to genetic and environmental changes.. Our earlier studies have shown differential effects of genetically inherited (GIO) and diet-inducedobesity (DIO) on DNA methylation in male germline.

Article Title: TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss.
Article Snippet: .. Briefly, the slices were dewaxed and subjected to gradient hydration and antigen retrieval, after which they were incubated with primary antibodies against LC3B (ABclonal, A19665; 1:100), BCL2 (Abcam, ab182858; 1:500), and TET2 (ABclonal, A1526; 1:100), followed by incubation with secondary and tertiary antibodies (Vector Laboratories, PK-6200). .. A DAB kit (Cell Signaling Technology, 8059S) was used to induce a color reaction.

Blocking Assay:

Membrane:

Image Search Results

(A and B) Naïve IgM + IgD + B cells from C57BL/6 mouse spleen ( n = 3) were cultured for 0, 24, 48, 72, or 96 h upon stimulation with E. coli 055:B5 LPS plus IL-4. (A) Left, cell Tet1, Tet2, Tet3, Aicda , and Prdm1 expression at the indicated time points. Right, intracellular TET2 expression in mouse naïve B cells, GC-like B cells, and plasma cells after 96 h culture. (B) Left, cell TET1, TET2, phosphorylated TET2 ( p -TET2), acetylated TET2 (Ac-TET2), TET3, AID, BLIMP-1, and β-ACTIN protein expression at indicated time points, as analyzed by specific immunoblots. Right, densitometry quantification of such immunoblotted signals is normalized to β-ACTIN levels. (C) Naïve huIgM + IgD + B cells from healthy humans ( n = 3) were cultured for 96 h upon stimulation with CD154, huIL-2, huIL-4, and huIL-21. Intracellular TET2 expression in human naïve B cells, GC-like B cells, and plasma cells. In truncated violin plots (A and C), each dot represents cell TET2 mean fluorescent intensity (MFI) from one in vitro B cell microculture. Top and bottom edges of each plot indicate the maximum and minimum MFI values, respectively; the line indicates the median. (D) Top, cell surface marker and intracellular TET2 expression profiles (heatmaps) of spleen and MLNs mononuclear cells in NP-CGG-immunized C57BL/6 mice ( n = 3) were integrated to create t-SNE maps and analyze intracellular TET2 expression in mouse naïve B cells, GC B cells, late GC B cells, plasmablasts, plasma cells, and swMBCs. Bottom, cell surface marker and intracellular TET2 expression profiles (heatmap) of human tonsil ( n = 3) mononuclear cells were merged to create t-SNE maps and analyze intracellular TET2 expression in human transitional B cells, naïve B cells, early GC B cells, GC B cells, double-negative (DN) B cells, plasma cells, and swMBCs. Statistical significance (A and C) was assessed using two-sided Student’s unpaired t test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Cell reports

Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

doi: 10.1016/j.celrep.2026.117370

Figure Lengend Snippet: (A and B) Naïve IgM + IgD + B cells from C57BL/6 mouse spleen ( n = 3) were cultured for 0, 24, 48, 72, or 96 h upon stimulation with E. coli 055:B5 LPS plus IL-4. (A) Left, cell Tet1, Tet2, Tet3, Aicda , and Prdm1 expression at the indicated time points. Right, intracellular TET2 expression in mouse naïve B cells, GC-like B cells, and plasma cells after 96 h culture. (B) Left, cell TET1, TET2, phosphorylated TET2 ( p -TET2), acetylated TET2 (Ac-TET2), TET3, AID, BLIMP-1, and β-ACTIN protein expression at indicated time points, as analyzed by specific immunoblots. Right, densitometry quantification of such immunoblotted signals is normalized to β-ACTIN levels. (C) Naïve huIgM + IgD + B cells from healthy humans ( n = 3) were cultured for 96 h upon stimulation with CD154, huIL-2, huIL-4, and huIL-21. Intracellular TET2 expression in human naïve B cells, GC-like B cells, and plasma cells. In truncated violin plots (A and C), each dot represents cell TET2 mean fluorescent intensity (MFI) from one in vitro B cell microculture. Top and bottom edges of each plot indicate the maximum and minimum MFI values, respectively; the line indicates the median. (D) Top, cell surface marker and intracellular TET2 expression profiles (heatmaps) of spleen and MLNs mononuclear cells in NP-CGG-immunized C57BL/6 mice ( n = 3) were integrated to create t-SNE maps and analyze intracellular TET2 expression in mouse naïve B cells, GC B cells, late GC B cells, plasmablasts, plasma cells, and swMBCs. Bottom, cell surface marker and intracellular TET2 expression profiles (heatmap) of human tonsil ( n = 3) mononuclear cells were merged to create t-SNE maps and analyze intracellular TET2 expression in human transitional B cells, naïve B cells, early GC B cells, GC B cells, double-negative (DN) B cells, plasma cells, and swMBCs. Statistical significance (A and C) was assessed using two-sided Student’s unpaired t test (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: anti-mo/hu-TET2 Ab , ABclonal , Cat. # A20365.

Techniques: Cell Culture, Expressing, Clinical Proteomics, Western Blot, In Vitro, Marker

Aicda Cre Tet2 +/+ ( n = 5) and Aicda Cre Tet2 fl/fl ( n = 5) mice were injected intraperitoneally (i.p.) with NP 16 -CGG on day 0, boosted on day 14, and euthanized on day 28. (A) Left, serum NP 4 -specific IgM, IgG1, and IgA, as measured by ELISAs (titers expressed as relative units, RUs). In floating bar plots, each dot represents one mouse. The top and bottom edges indicate the full range of the data. Right, NP 4 -specific IgM, IgG1, and IgA ASCs in spleen and BM, as analyzed and enumerated by specific ELISPOTs. (B) IgM + , IgD + , and IgA + cells in Peyer’s patches (Scale bars: 100 μm). (C) GC B220 + GL7 + CD95 + IgG1 + B cells, B220 + IgG1 + B cells, B220 + CD138 + plasmablasts, NP 5 -specific GC B220 + IgM – IgD – IgG1 + B cells and NP 5 -specific CD38 + IgG1 + swMBCs among mononuclear cells in spleen. (D) B cell Tet2, Aicda, Prdm1, Irf4 , and Bach2 transcript expression in spleen, as analyzed by qRT-PCR. (E) Transduced Tet2 +/+ and Tet2 −/− B cells were cultured for 96 h upon stimulation with LPS plus IL-4. Left, CD19 + IgG1 + B cells and CD19 + CD138 + plasmablasts among pre-gated Tet2 −/− GFP + cells transduced by pMIG-Empty , pMIG-Aicda , or pMIG-Prdm1 retroviral vector (left, n = 3) or among Tet2 +/+ GFP + cells transduced by pMIG-Empty or pMIG-Tet2 retroviral vectors (right, n = 3). (F) Somatic point-mutations in IgG1 + B cell V1-72DJ H -Cγ1 transcripts of NP-CGG-immunized mice. Pie charts: slices depict proportions of V1-72DJ H -Cγ1 transcripts carrying different numbers of point-mutations; slice gray gradients depict increasing numbers of point-mutations (from 0 to more than 12 per transcript); the overall mutation frequency is listed below each pie chart ( Aicda Cre Tet2 +/+ : 2.51 × 10 −2 change/base vs. Aicda Cre Tet2 fl/fl : 0.42 × 10 −2 change/base). Histograms depict means of total, S, and R V1-72 mutation frequencies (change/base) in FR1, CDR1, FR2, CDR2, and FR3 of V1-72DJ H -Cγ1 transcripts. Boxplots depict overall V1-72 point-mutation in V1-72DJ H -Cγ1 transcripts. In boxplots, data points are displayed as individual dots, each dot representing a single sequence. Box includes the 2 nd and 3 rd quartiles (top 75% to bottom 25%) of data, line indicates median, and cross indicates the geometrical mean of data, whiskers show the spread of data (95%–5%), and dots above and below whiskers are outliers. (G and H) Naïve IgM + IgD + B cells from Aicda Cre Tet2 +/+ ( n = 3) and Aicda Cre Tet2 fl/fl ( n = 3) mice spleen were cultured for 72 h upon stimulation with LPS plus IL-4. (G) Intracellular AID and BLIMP-1 expression were analyzed by flow cytometry. (H) AID, BLIMP-1, and β-ACTIN protein expression as analyzed by specific immunoblots. Statistical significance (A, C, E, and G) was assessed using two-sided Student’s unpaired t test (ns, not significant; ** p < 0.01, *** p < 0.001).

Journal: Cell reports

Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

doi: 10.1016/j.celrep.2026.117370

Figure Lengend Snippet: Aicda Cre Tet2 +/+ ( n = 5) and Aicda Cre Tet2 fl/fl ( n = 5) mice were injected intraperitoneally (i.p.) with NP 16 -CGG on day 0, boosted on day 14, and euthanized on day 28. (A) Left, serum NP 4 -specific IgM, IgG1, and IgA, as measured by ELISAs (titers expressed as relative units, RUs). In floating bar plots, each dot represents one mouse. The top and bottom edges indicate the full range of the data. Right, NP 4 -specific IgM, IgG1, and IgA ASCs in spleen and BM, as analyzed and enumerated by specific ELISPOTs. (B) IgM + , IgD + , and IgA + cells in Peyer’s patches (Scale bars: 100 μm). (C) GC B220 + GL7 + CD95 + IgG1 + B cells, B220 + IgG1 + B cells, B220 + CD138 + plasmablasts, NP 5 -specific GC B220 + IgM – IgD – IgG1 + B cells and NP 5 -specific CD38 + IgG1 + swMBCs among mononuclear cells in spleen. (D) B cell Tet2, Aicda, Prdm1, Irf4 , and Bach2 transcript expression in spleen, as analyzed by qRT-PCR. (E) Transduced Tet2 +/+ and Tet2 −/− B cells were cultured for 96 h upon stimulation with LPS plus IL-4. Left, CD19 + IgG1 + B cells and CD19 + CD138 + plasmablasts among pre-gated Tet2 −/− GFP + cells transduced by pMIG-Empty , pMIG-Aicda , or pMIG-Prdm1 retroviral vector (left, n = 3) or among Tet2 +/+ GFP + cells transduced by pMIG-Empty or pMIG-Tet2 retroviral vectors (right, n = 3). (F) Somatic point-mutations in IgG1 + B cell V1-72DJ H -Cγ1 transcripts of NP-CGG-immunized mice. Pie charts: slices depict proportions of V1-72DJ H -Cγ1 transcripts carrying different numbers of point-mutations; slice gray gradients depict increasing numbers of point-mutations (from 0 to more than 12 per transcript); the overall mutation frequency is listed below each pie chart ( Aicda Cre Tet2 +/+ : 2.51 × 10 −2 change/base vs. Aicda Cre Tet2 fl/fl : 0.42 × 10 −2 change/base). Histograms depict means of total, S, and R V1-72 mutation frequencies (change/base) in FR1, CDR1, FR2, CDR2, and FR3 of V1-72DJ H -Cγ1 transcripts. Boxplots depict overall V1-72 point-mutation in V1-72DJ H -Cγ1 transcripts. In boxplots, data points are displayed as individual dots, each dot representing a single sequence. Box includes the 2 nd and 3 rd quartiles (top 75% to bottom 25%) of data, line indicates median, and cross indicates the geometrical mean of data, whiskers show the spread of data (95%–5%), and dots above and below whiskers are outliers. (G and H) Naïve IgM + IgD + B cells from Aicda Cre Tet2 +/+ ( n = 3) and Aicda Cre Tet2 fl/fl ( n = 3) mice spleen were cultured for 72 h upon stimulation with LPS plus IL-4. (G) Intracellular AID and BLIMP-1 expression were analyzed by flow cytometry. (H) AID, BLIMP-1, and β-ACTIN protein expression as analyzed by specific immunoblots. Statistical significance (A, C, E, and G) was assessed using two-sided Student’s unpaired t test (ns, not significant; ** p < 0.01, *** p < 0.001).

Article Snippet: anti-mo/hu-TET2 Ab , ABclonal , Cat. # A20365.

Techniques: Injection, Expressing, Quantitative RT-PCR, Cell Culture, Retroviral, Plasmid Preparation, Mutagenesis, Sequencing, Flow Cytometry, Western Blot

Naïve IgM + IgD + B cells from Aicda Cre Ogt +/+ and Aidca Cre Ogt fl/fl mice spleen ( n = 3, A–C) or Aicda Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl mice spleen ( n = 3, B and G) were cultured for 72 h or 96 h upon application of GC-like cell-inducing stimuli. (A) Left, CD19 + IgG1 + , CD19 + IgG3 + or CD19 + IgA + B cells and CD19 + CD138 + plasmablasts. Right, intracellular AID and BLIMP-1 expression in B cells stimulated with LPS plus IL-4 after 96 h microculture. (B) Methylated and hydroxymethylated DNA levels in promoter and enhancer regions of Aicda and Prdm1 gene loci in Aicda Cre Tet2 +/+ , Aicda Cre Tet2 fl/fl (top), Aicda Cre Ogt +/+ , and Aicda Cre Ogt fl/fl (bottom) cells after 72 h microculture, as analyzed by MeDIP-qPCR and hMeDIP-qPCR. (C) Expression of TET2 and O -GlcNAcylated TET2 in Aidca Cre Ogt +/+ and Aidca Cre Ogt fl/fl cells after 96 h microculture. (D) Naïve IgM + IgD + B cells from C57BL/6 ( n = 3) mice spleen or healthy humans ( n = 3) were cultured for 0, 24, 48, 72, and 96 h upon stimulation with LPS plus IL-4 or CpG (ODN 2395) plus huIL-2, huIL-4, and huIL-21, respectively. Expression of TET2 and O -GlcNAcylated TET2 in mouse and human B cells at the indicated time points. (E) TET2 O -GlcNAcylation sites in mouse B cells, as predicted by YinOYang and O -GlcNAcPRED-DL. (F) Naïve IgM + IgD + B cells from C57BL/6 mice ( n = 3) spleen were cultured for 72 h upon stimulation with LPS plus IL-4. TET2 or OGT interaction with TET2, OGT, PROSER1, HDAC1, and CREBBP in cell nuclei, as analyzed by specific immunoblots. (G) Aidca Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl B cell OGT (left) and O -GlcNAc H2B-S112 (right) levels after 72 h microculture, as expressed in promoter and enhancer regions of Aicda and Prdm1 gene loci, as analyzed by ChIP-qPCR. Statistical significance (B and G) was assessed using two-sided Student’s unpaired t test (** p < 0.01, *** p < 0.001).

Journal: Cell reports

Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

doi: 10.1016/j.celrep.2026.117370

Figure Lengend Snippet: Naïve IgM + IgD + B cells from Aicda Cre Ogt +/+ and Aidca Cre Ogt fl/fl mice spleen ( n = 3, A–C) or Aicda Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl mice spleen ( n = 3, B and G) were cultured for 72 h or 96 h upon application of GC-like cell-inducing stimuli. (A) Left, CD19 + IgG1 + , CD19 + IgG3 + or CD19 + IgA + B cells and CD19 + CD138 + plasmablasts. Right, intracellular AID and BLIMP-1 expression in B cells stimulated with LPS plus IL-4 after 96 h microculture. (B) Methylated and hydroxymethylated DNA levels in promoter and enhancer regions of Aicda and Prdm1 gene loci in Aicda Cre Tet2 +/+ , Aicda Cre Tet2 fl/fl (top), Aicda Cre Ogt +/+ , and Aicda Cre Ogt fl/fl (bottom) cells after 72 h microculture, as analyzed by MeDIP-qPCR and hMeDIP-qPCR. (C) Expression of TET2 and O -GlcNAcylated TET2 in Aidca Cre Ogt +/+ and Aidca Cre Ogt fl/fl cells after 96 h microculture. (D) Naïve IgM + IgD + B cells from C57BL/6 ( n = 3) mice spleen or healthy humans ( n = 3) were cultured for 0, 24, 48, 72, and 96 h upon stimulation with LPS plus IL-4 or CpG (ODN 2395) plus huIL-2, huIL-4, and huIL-21, respectively. Expression of TET2 and O -GlcNAcylated TET2 in mouse and human B cells at the indicated time points. (E) TET2 O -GlcNAcylation sites in mouse B cells, as predicted by YinOYang and O -GlcNAcPRED-DL. (F) Naïve IgM + IgD + B cells from C57BL/6 mice ( n = 3) spleen were cultured for 72 h upon stimulation with LPS plus IL-4. TET2 or OGT interaction with TET2, OGT, PROSER1, HDAC1, and CREBBP in cell nuclei, as analyzed by specific immunoblots. (G) Aidca Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl B cell OGT (left) and O -GlcNAc H2B-S112 (right) levels after 72 h microculture, as expressed in promoter and enhancer regions of Aicda and Prdm1 gene loci, as analyzed by ChIP-qPCR. Statistical significance (B and G) was assessed using two-sided Student’s unpaired t test (** p < 0.01, *** p < 0.001).

Article Snippet: anti-mo/hu-TET2 Ab , ABclonal , Cat. # A20365.

Techniques: Cell Culture, Expressing, Methylation, Methylated DNA Immunoprecipitation, Western Blot, ChIP-qPCR

(A) TET2 isoform 2 (green)-DNA (gray)-OGT (yellow) complex assembly at Aicda Area IV (top, iPTM = 0.65) and Prdm1 Area II (bottom, iPTM = 0.66), as predicted by AlphaFold 3.0; depicted are TET2 conserved signature HxD catalytic motif (purple) and TET2 C-terminal-end OGT-binding site (dark blue). (B) Schematic of pMIG-Tet2 WT , pMIG-Tet2 HxD-mut , pMIG-Tet2 OGT -Δ mut , and pMIG-Tet2 HxD-mut/OGT -Δ mut constructs. (C) Tet2 −/− B cells ( n = 3) were transduced to induce expression of pMIG-Empty vector , pMIG-Tet2 WT , pMIG-Tet2 HxD-mut , pMIG-Tet2 OGT -Δ mut , or pMIG-Tet2 HxD-mut/OGT -Δ mut and cultured for 96 h upon stimulation with LPS plus IL-4. TET2 expression in these B cells, as analyzed by specific immunoblots. CD19 + IgG1 + B cells, CD19 + CD138 + plasmablasts, and intracellular TET2 expression among transduced GFP + Tet2 −/− cells (pre-gated). Statistical significance was assessed using two-sided Student’s unpaired t test (ns, not significant; *** p < 0.001).

Journal: Cell reports

Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

doi: 10.1016/j.celrep.2026.117370

Figure Lengend Snippet: (A) TET2 isoform 2 (green)-DNA (gray)-OGT (yellow) complex assembly at Aicda Area IV (top, iPTM = 0.65) and Prdm1 Area II (bottom, iPTM = 0.66), as predicted by AlphaFold 3.0; depicted are TET2 conserved signature HxD catalytic motif (purple) and TET2 C-terminal-end OGT-binding site (dark blue). (B) Schematic of pMIG-Tet2 WT , pMIG-Tet2 HxD-mut , pMIG-Tet2 OGT -Δ mut , and pMIG-Tet2 HxD-mut/OGT -Δ mut constructs. (C) Tet2 −/− B cells ( n = 3) were transduced to induce expression of pMIG-Empty vector , pMIG-Tet2 WT , pMIG-Tet2 HxD-mut , pMIG-Tet2 OGT -Δ mut , or pMIG-Tet2 HxD-mut/OGT -Δ mut and cultured for 96 h upon stimulation with LPS plus IL-4. TET2 expression in these B cells, as analyzed by specific immunoblots. CD19 + IgG1 + B cells, CD19 + CD138 + plasmablasts, and intracellular TET2 expression among transduced GFP + Tet2 −/− cells (pre-gated). Statistical significance was assessed using two-sided Student’s unpaired t test (ns, not significant; *** p < 0.001).

Article Snippet: anti-mo/hu-TET2 Ab , ABclonal , Cat. # A20365.

Techniques: Binding Assay, Construct, Expressing, Plasmid Preparation, Cell Culture, Western Blot

Humanized THX mice were fed nil ( n = 5) or dimethyl fumarate (DMF, 0.5 mg/mL, n = 5) on day 0 and continued thereafter. (A-E) Nil-fed and DMF-fed THX mice were injected i.p. with NP 16 -CGG on day 7, boosted on days 14, 21, and 28, and euthanized on day 35. (A) Left, schematic of NP 16 -CGG immunization. Right, serum NP 4 -specific huIgM, huIgG1, and huIgA, as measured by ELISAs (titers expressed as RUs). (B) Intracellular TET2, AID, and BLIMP-1 expression among pre-gated huCD45 + CD19 + B cells in spleen. (C) Left, class-switched memory huCD27 + CD38 − B cells and huCD27 + CD38 + plasmablasts among pre-gated huCD19 + IgG + or huCD19 + IgA + cells in spleen. Right, NP 5 -specific huIgG + and huIgA + B cells among pre-gated huCD45 + CD19 + cells in spleen. (D) Left, memory huCD27 + CD38 − B cells and huCD27 + CD38 + plasmablasts among pre-gated huCD19 + B cells in spleen and BM. Right, huIgG + and huIgA + B cells among pre-gated huCD45 + CD19 + cells in spleen and BM. (E) Somatic point-mutations in huIgG1 + B cell huV3DJ H -Cγ1 transcripts of NP-CGG-immunized THX mice. Left, pie charts depict proportions of huV3DJ H -Cγ1 transcripts carrying different numbers of point-mutations; the overall mutation frequency is listed below each pie chart (Nil-fed THX mice: 2.2 × 10 −2 change/base vs. DMF-fed THX mice: 0.9 × 10 −2 change/base). Center, histograms depict means of total, S, and R huV3 mutation frequencies (change/base) in FR1, CDR1, FR2, CDR2, and FR3 of huV3DJH-Cγ1. Boxplots depict overall V1-72 point-mutation frequency in huV3DJ H -Cγ1 transcripts. Right, huV3DJ H -Cγ1 B cell clones and intraclonal diversification, as depicted by TreeMaps and phylogenetic trees, respectively. An individual rectangle or square (unique color) Area reflects the human B cell clone size. (F) Naïve huIgM + IgD + B cells from healthy humans ( n = 3) were cultured for 72 h or 96 h upon stimulation with CpG plus huIL-2, huIL-4, and huIL-21 in the presence of nil or vitamin C (200 μM). Left, huIgG + B cells, huIgA + B cells, and huCD27 + CD38 + plasmablasts among pre-gated huCD19 + cells after 96 h culture. Center, cell AICDA and PRDM1 expression after 72 h of culture. Right, methylated and hydroxymethylated DNA in promoter and enhancer regions of AICDA and PRDM1 gene loci after 72 h culture in human B cells. Statistical significance (A and C–F) was assessed using two-sided Student’s unpaired t test (ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Cell reports

Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

doi: 10.1016/j.celrep.2026.117370

Figure Lengend Snippet: Humanized THX mice were fed nil ( n = 5) or dimethyl fumarate (DMF, 0.5 mg/mL, n = 5) on day 0 and continued thereafter. (A-E) Nil-fed and DMF-fed THX mice were injected i.p. with NP 16 -CGG on day 7, boosted on days 14, 21, and 28, and euthanized on day 35. (A) Left, schematic of NP 16 -CGG immunization. Right, serum NP 4 -specific huIgM, huIgG1, and huIgA, as measured by ELISAs (titers expressed as RUs). (B) Intracellular TET2, AID, and BLIMP-1 expression among pre-gated huCD45 + CD19 + B cells in spleen. (C) Left, class-switched memory huCD27 + CD38 − B cells and huCD27 + CD38 + plasmablasts among pre-gated huCD19 + IgG + or huCD19 + IgA + cells in spleen. Right, NP 5 -specific huIgG + and huIgA + B cells among pre-gated huCD45 + CD19 + cells in spleen. (D) Left, memory huCD27 + CD38 − B cells and huCD27 + CD38 + plasmablasts among pre-gated huCD19 + B cells in spleen and BM. Right, huIgG + and huIgA + B cells among pre-gated huCD45 + CD19 + cells in spleen and BM. (E) Somatic point-mutations in huIgG1 + B cell huV3DJ H -Cγ1 transcripts of NP-CGG-immunized THX mice. Left, pie charts depict proportions of huV3DJ H -Cγ1 transcripts carrying different numbers of point-mutations; the overall mutation frequency is listed below each pie chart (Nil-fed THX mice: 2.2 × 10 −2 change/base vs. DMF-fed THX mice: 0.9 × 10 −2 change/base). Center, histograms depict means of total, S, and R huV3 mutation frequencies (change/base) in FR1, CDR1, FR2, CDR2, and FR3 of huV3DJH-Cγ1. Boxplots depict overall V1-72 point-mutation frequency in huV3DJ H -Cγ1 transcripts. Right, huV3DJ H -Cγ1 B cell clones and intraclonal diversification, as depicted by TreeMaps and phylogenetic trees, respectively. An individual rectangle or square (unique color) Area reflects the human B cell clone size. (F) Naïve huIgM + IgD + B cells from healthy humans ( n = 3) were cultured for 72 h or 96 h upon stimulation with CpG plus huIL-2, huIL-4, and huIL-21 in the presence of nil or vitamin C (200 μM). Left, huIgG + B cells, huIgA + B cells, and huCD27 + CD38 + plasmablasts among pre-gated huCD19 + cells after 96 h culture. Center, cell AICDA and PRDM1 expression after 72 h of culture. Right, methylated and hydroxymethylated DNA in promoter and enhancer regions of AICDA and PRDM1 gene loci after 72 h culture in human B cells. Statistical significance (A and C–F) was assessed using two-sided Student’s unpaired t test (ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: anti-mo/hu-TET2 Ab , ABclonal , Cat. # A20365.

Techniques: Injection, Expressing, Mutagenesis, Clone Assay, Cell Culture, Methylation

(A-D) Tcrβ −/− Tcrδ −/− mice were injected i.p. with nil (PBS, n = 7) or vitamin C (Vit C, 80 mg per mice, n = 7) on days 1, 1, 3, and 5. Nil-treated and vitamin C-treated Tcrβ −/− Tcrδ −/− mice were immunized i.p. with NP 0.4 -LPS on day 0, boosted on days 14 and 23, and euthanized on day 44. (A) Top left, schematic of nil or vitamin C administration and NP 0.4 -LPS immunization. Top center, sera were collected on days 7, 14, 23, 33, and 44 and analyzed for NP 4 - or LPS-specific IgM, IgG3, and IgG2b, as measured by ELISAs (titers expressed as RUs). Truncated violin plots depict NP 4 - or LPS-specific IgM, IgG3, and IgG2b titers through day 44. Top right, floating bar plots depict NP 4 - or LPS-specific antibody titers at day 33. Bottom right, GC GL7 + CD95 + B cells, class-switched GC GL7 + CD95 + IgG3 + and GL7 + CD95 + IgG2b + B cells among pre-gated CD19 + cells in MLNs (left), NP 5 -specific IgG3 + and IgG2b + B cells among pre-gated CD19 + cells in spleen (center), and CD19 + CD138 + plasmablasts among mononuclear cells in MLNs (right). (B) NP 4 -specific IgM, IgG3, and IgG2b ASCs in spleen and BM, as analyzed and enumerated by specific ELISPOTs. (C) Somatic point-mutations in IgG3 + B cell V1-72DJ H -Cγ3 and IgG2b + B cell V1-72DJ H -Cγ2b transcripts. Left, pie charts depict proportions of V1-72DJ H -Cγ3 and V1-72DJ H -Cγ2b transcripts carrying different numbers of point-mutations; the overall mutation frequency is listed below each pie chart (V1-72DJ H -Cγ3: nil, 0.65 × 10 −2 change/base vs. vitamin C, 2.19 × 10 −2 change/base; V1-72DJ H -Cγ2b: nil, 0.71 × 10 −2 change/base vs. vitamin C, 1.35 × 10 −2 change/base). Center, histograms depict means of total, S, and R V1-72 mutation frequencies (change/base) in FR1, CDR1, FR2, CDR2, and FR3 of V1-72DJ H -Cγ3 and V1-72DJ H -Cγ2b transcripts. Right, boxplots depict overall V1-72 point-mutation frequency in V1-72DJ H -Cγ3 and V1-72DJ H -Cγ2b transcripts. (D) Intracellular TET2, AID, and BLIMP-1 expression among pre-gated CD19 + cells in spleen. (E) Naïve IgM + IgD + B cells from C57BL/6 mice ( n = 3) were cultured for 72 h or 96 h upon stimulation with LPS plus IL-4 in the presence of nil or vitamin C (200 μM). Left, flow cytometry plots from one in vitro B cell microculture representative of 3 cell microcultures. CD19 + IgG1 + B cells and CD19 + CD138 + plasmablasts after 96 h culture. Top right, recruited TET2 and O -GlcNAcylation H2B-S112 levels in Aicda and Prdm1 promoter and enhancer regions in mouse B cells after 72 h culture. Bottom right, cell methylated and hydroxymethylated DNA in Aicda and Prdm1 promoter and enhancer regions after 72 h culture. Statistical significance (A, C, and E) was assessed using two-sided Student’s unpaired t test (ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Cell reports

Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

doi: 10.1016/j.celrep.2026.117370

Figure Lengend Snippet: (A-D) Tcrβ −/− Tcrδ −/− mice were injected i.p. with nil (PBS, n = 7) or vitamin C (Vit C, 80 mg per mice, n = 7) on days 1, 1, 3, and 5. Nil-treated and vitamin C-treated Tcrβ −/− Tcrδ −/− mice were immunized i.p. with NP 0.4 -LPS on day 0, boosted on days 14 and 23, and euthanized on day 44. (A) Top left, schematic of nil or vitamin C administration and NP 0.4 -LPS immunization. Top center, sera were collected on days 7, 14, 23, 33, and 44 and analyzed for NP 4 - or LPS-specific IgM, IgG3, and IgG2b, as measured by ELISAs (titers expressed as RUs). Truncated violin plots depict NP 4 - or LPS-specific IgM, IgG3, and IgG2b titers through day 44. Top right, floating bar plots depict NP 4 - or LPS-specific antibody titers at day 33. Bottom right, GC GL7 + CD95 + B cells, class-switched GC GL7 + CD95 + IgG3 + and GL7 + CD95 + IgG2b + B cells among pre-gated CD19 + cells in MLNs (left), NP 5 -specific IgG3 + and IgG2b + B cells among pre-gated CD19 + cells in spleen (center), and CD19 + CD138 + plasmablasts among mononuclear cells in MLNs (right). (B) NP 4 -specific IgM, IgG3, and IgG2b ASCs in spleen and BM, as analyzed and enumerated by specific ELISPOTs. (C) Somatic point-mutations in IgG3 + B cell V1-72DJ H -Cγ3 and IgG2b + B cell V1-72DJ H -Cγ2b transcripts. Left, pie charts depict proportions of V1-72DJ H -Cγ3 and V1-72DJ H -Cγ2b transcripts carrying different numbers of point-mutations; the overall mutation frequency is listed below each pie chart (V1-72DJ H -Cγ3: nil, 0.65 × 10 −2 change/base vs. vitamin C, 2.19 × 10 −2 change/base; V1-72DJ H -Cγ2b: nil, 0.71 × 10 −2 change/base vs. vitamin C, 1.35 × 10 −2 change/base). Center, histograms depict means of total, S, and R V1-72 mutation frequencies (change/base) in FR1, CDR1, FR2, CDR2, and FR3 of V1-72DJ H -Cγ3 and V1-72DJ H -Cγ2b transcripts. Right, boxplots depict overall V1-72 point-mutation frequency in V1-72DJ H -Cγ3 and V1-72DJ H -Cγ2b transcripts. (D) Intracellular TET2, AID, and BLIMP-1 expression among pre-gated CD19 + cells in spleen. (E) Naïve IgM + IgD + B cells from C57BL/6 mice ( n = 3) were cultured for 72 h or 96 h upon stimulation with LPS plus IL-4 in the presence of nil or vitamin C (200 μM). Left, flow cytometry plots from one in vitro B cell microculture representative of 3 cell microcultures. CD19 + IgG1 + B cells and CD19 + CD138 + plasmablasts after 96 h culture. Top right, recruited TET2 and O -GlcNAcylation H2B-S112 levels in Aicda and Prdm1 promoter and enhancer regions in mouse B cells after 72 h culture. Bottom right, cell methylated and hydroxymethylated DNA in Aicda and Prdm1 promoter and enhancer regions after 72 h culture. Statistical significance (A, C, and E) was assessed using two-sided Student’s unpaired t test (ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: anti-mo/hu-TET2 Ab , ABclonal , Cat. # A20365.

Techniques: Injection, Mutagenesis, Expressing, Cell Culture, Flow Cytometry, In Vitro, Methylation

(A) Red blood cell count, hemoglobin, and monocyte count of lethally irradiated WT or Gsdmd ⁻/⁻ recipients transplanted with HSPCs from WT or Tet2 -/- donors at 9 months post-transplant. n = 6 in each group. (B) Flow cytometry analyses of Ly6G⁻Ly6C⁺ monocyte frequencies in CD11b + peripheral blood mononuclear cells (PBMCs) and bone marrow (BM) from indicated transplant groups at 9 months post-transplant. n = 4 in each group. (C) Quantification of spleen weights from the indicated transplant groups at 9 months post-transplant. n = 4 in each group. (D) Flow cytometric analysis of splenic cellular composition showing frequencies of CD11b⁺ myeloid cells and CD71⁺ erythroid precursors from indicated transplant groups. n = 4 in each group. (E) Representative hematoxylin and eosin (H&E) staining of bone marrow, spleen, and liver sections from the indicated transplant groups. Scale bars: 300 μm. (F) Whole-mount confocal imaging of murine femurs showing gasdermin D N-terminal domain (Gsdmd-NTD, magenta) and CD271⁺ mesenchymal stromal cells (MSCs, green) from indicated transplant groups. Arrows indicate punctate membrane-associated Gsdmd-NTD signals indicative of active pyroptosis. Scale bars: 50 μm. (G) High plex Xenium (Xenium 5K) spatial transcriptomic analysis of bone marrow sections from indicated transplant groups. The distributions of monocytes/macrophages relative to stromal cell and T cell populations are highlighted on the right. (H) Cell-cell interaction network analysis demonstrating stromal-macrophage-T cell signaling interactions in each transplant group. The thickness of the connecting lines indicates the strength of the interaction. (I) Schematic of an in vitro co-culture system. The right panels illustrate the increased proportion of CellTrace⁺ Tet2 ⁻/⁻ macrophages with high MHC-II expression. (J) Quantification of CellTrace⁺ Tet2 ⁻/⁻ macrophages following co-culture with unprimed or primed wild-type or Gsdmd ⁻/⁻ MSCs. n = 3 in each group. (K) Flow cytometric analysis of MHC-II expression on Tet2 ⁻/⁻ macrophages following co-culture with unprimed or primed MSCs. n = 3 in each group. All the error bars represent the SEM of the mean. Comparisons among multiple groups were evaluated using a 1-way ANOVA. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. ns: not significant.

Journal: bioRxiv

Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis

doi: 10.64898/2026.05.01.722102

Figure Lengend Snippet: (A) Red blood cell count, hemoglobin, and monocyte count of lethally irradiated WT or Gsdmd ⁻/⁻ recipients transplanted with HSPCs from WT or Tet2 -/- donors at 9 months post-transplant. n = 6 in each group. (B) Flow cytometry analyses of Ly6G⁻Ly6C⁺ monocyte frequencies in CD11b + peripheral blood mononuclear cells (PBMCs) and bone marrow (BM) from indicated transplant groups at 9 months post-transplant. n = 4 in each group. (C) Quantification of spleen weights from the indicated transplant groups at 9 months post-transplant. n = 4 in each group. (D) Flow cytometric analysis of splenic cellular composition showing frequencies of CD11b⁺ myeloid cells and CD71⁺ erythroid precursors from indicated transplant groups. n = 4 in each group. (E) Representative hematoxylin and eosin (H&E) staining of bone marrow, spleen, and liver sections from the indicated transplant groups. Scale bars: 300 μm. (F) Whole-mount confocal imaging of murine femurs showing gasdermin D N-terminal domain (Gsdmd-NTD, magenta) and CD271⁺ mesenchymal stromal cells (MSCs, green) from indicated transplant groups. Arrows indicate punctate membrane-associated Gsdmd-NTD signals indicative of active pyroptosis. Scale bars: 50 μm. (G) High plex Xenium (Xenium 5K) spatial transcriptomic analysis of bone marrow sections from indicated transplant groups. The distributions of monocytes/macrophages relative to stromal cell and T cell populations are highlighted on the right. (H) Cell-cell interaction network analysis demonstrating stromal-macrophage-T cell signaling interactions in each transplant group. The thickness of the connecting lines indicates the strength of the interaction. (I) Schematic of an in vitro co-culture system. The right panels illustrate the increased proportion of CellTrace⁺ Tet2 ⁻/⁻ macrophages with high MHC-II expression. (J) Quantification of CellTrace⁺ Tet2 ⁻/⁻ macrophages following co-culture with unprimed or primed wild-type or Gsdmd ⁻/⁻ MSCs. n = 3 in each group. (K) Flow cytometric analysis of MHC-II expression on Tet2 ⁻/⁻ macrophages following co-culture with unprimed or primed MSCs. n = 3 in each group. All the error bars represent the SEM of the mean. Comparisons among multiple groups were evaluated using a 1-way ANOVA. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. ns: not significant.

Article Snippet: Tet2 floxed mice (#017573), Vav-iCre mice (#008610), and miR-146a knockout mice (#016239) were purchased from the Jackson Laboratory.

Techniques: Cell Characterization, Irradiation, Flow Cytometry, Staining, Imaging, Membrane, In Vitro, Co-Culture Assay, Expressing

(A) UMAP visualization of single-cell RNA sequencing data from total bone marrow cells (after erythroid lysis) from WT to WT, Tet2 ⁻/⁻ to WT, and Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ recipients at 9 months post-transplant. The black circle indicates macrophage populations; the red circle indicates T-cell populations. (B-C) UMAP visualization of mature (B) and early differentiated (C) macrophage subpopulations across indicated transplant groups in A. (D) Venn diagram showing overlap of differentially expressed genes in macrophages from Tet2 ⁻/⁻ to WT recipients compared with WT controls, and genes reversed in Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ recipients. (E) Gene Ontology pathway enrichment analysis of differentially expressed genes in macrophages that are upregulated in Tet2 ⁻/⁻ to WT recipients and reversed in Gsdmd ⁻/⁻ recipients. Dot size represents gene count; color represents adjusted p-value. (F) UMAP visualization of T-cell subpopulations across indicated transplant groups in a, with subclusters annotated by cell type and differentiation state. The T FH -like population is highlighted in a red circle. (G-H) Expression of canonical T FH markers ( Bcl6 , Cxcr5 , Icos , Il21 , Cd40l ), interferon-responsive genes ( Ifng , Tbx21 , Cxcr3 ) (G), and T FH -like-specific genes (H) across T-cell subpopulations. (I) Left: Heatmap of cluster-wise mean cytokine expression in bone marrow from WT to WT, Tet2 ⁻/⁻ to WT, and Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ recipients (n = 4 per group). The color scale represents z-scored expression values. Right: Hierarchical clustering dendrogram of cytokines based on expression patterns across all samples.

Journal: bioRxiv

Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis

doi: 10.64898/2026.05.01.722102

Figure Lengend Snippet: (A) UMAP visualization of single-cell RNA sequencing data from total bone marrow cells (after erythroid lysis) from WT to WT, Tet2 ⁻/⁻ to WT, and Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ recipients at 9 months post-transplant. The black circle indicates macrophage populations; the red circle indicates T-cell populations. (B-C) UMAP visualization of mature (B) and early differentiated (C) macrophage subpopulations across indicated transplant groups in A. (D) Venn diagram showing overlap of differentially expressed genes in macrophages from Tet2 ⁻/⁻ to WT recipients compared with WT controls, and genes reversed in Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ recipients. (E) Gene Ontology pathway enrichment analysis of differentially expressed genes in macrophages that are upregulated in Tet2 ⁻/⁻ to WT recipients and reversed in Gsdmd ⁻/⁻ recipients. Dot size represents gene count; color represents adjusted p-value. (F) UMAP visualization of T-cell subpopulations across indicated transplant groups in a, with subclusters annotated by cell type and differentiation state. The T FH -like population is highlighted in a red circle. (G-H) Expression of canonical T FH markers ( Bcl6 , Cxcr5 , Icos , Il21 , Cd40l ), interferon-responsive genes ( Ifng , Tbx21 , Cxcr3 ) (G), and T FH -like-specific genes (H) across T-cell subpopulations. (I) Left: Heatmap of cluster-wise mean cytokine expression in bone marrow from WT to WT, Tet2 ⁻/⁻ to WT, and Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ recipients (n = 4 per group). The color scale represents z-scored expression values. Right: Hierarchical clustering dendrogram of cytokines based on expression patterns across all samples.

Article Snippet: Tet2 floxed mice (#017573), Vav-iCre mice (#008610), and miR-146a knockout mice (#016239) were purchased from the Jackson Laboratory.

Techniques: Single Cell, RNA Sequencing, Lysis, Expressing

(A) Peripheral blood monocyte count in Tet2 ⁻/⁻ to WT chimeras treated with anti-CD40L blocking antibody or IgG control for 3 months. Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ chimeras are shown for comparison. n = 7 in WT to WT group; n = 7 in Tet2 ⁻/⁻ to WT IgG group; n = 8 in Tet2 ⁻/⁻ to WT anti-CD40L group; n = 12 in Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ group. (B) Representative flow cytometry plots showing Ly6C Hi inflammatory monocyte frequencies in peripheral blood from indicated treatment groups. (C) Quantification of Ly6C Hi inflammatory monocytes from the indicated groups. n = 3 in each group. (D) Representative flow cytometry plots of Ly6G and Ly6C bone marrow cells in the indicated groups. (E) Quantification of Ly6CHi inflammatory monocyte frequency in bone marrow across the indicated groups. (F) Quantification of Ly6CHi inflammatory monocyte frequency in spleen across different groups. (G) Spleen to body weight ratios in the indicated groups. n = 3 in each group. (H) Histopathological analysis of liver (top row), spleen (middle row), and bone marrow (bottom row) from the indicated chimeras. Tet2⁻/⁻ to WT IgG-treated mice display hepatic myeloid infiltration (dashed outline), splenic white pulp effacement by expanding myeloid elements, and hypercellular bone marrow with increased myeloid-to-erythroid ratio. Scale bars: liver and spleen, 300 μm; bone marrow, 100 μm. All the error bars represent the SEM of the mean. Comparisons among multiple groups were evaluated using a 1-way ANOVA. *p<0.05, **p<0.01. ns: not significant.

Journal: bioRxiv

Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis

doi: 10.64898/2026.05.01.722102

Figure Lengend Snippet: (A) Peripheral blood monocyte count in Tet2 ⁻/⁻ to WT chimeras treated with anti-CD40L blocking antibody or IgG control for 3 months. Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ chimeras are shown for comparison. n = 7 in WT to WT group; n = 7 in Tet2 ⁻/⁻ to WT IgG group; n = 8 in Tet2 ⁻/⁻ to WT anti-CD40L group; n = 12 in Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ group. (B) Representative flow cytometry plots showing Ly6C Hi inflammatory monocyte frequencies in peripheral blood from indicated treatment groups. (C) Quantification of Ly6C Hi inflammatory monocytes from the indicated groups. n = 3 in each group. (D) Representative flow cytometry plots of Ly6G and Ly6C bone marrow cells in the indicated groups. (E) Quantification of Ly6CHi inflammatory monocyte frequency in bone marrow across the indicated groups. (F) Quantification of Ly6CHi inflammatory monocyte frequency in spleen across different groups. (G) Spleen to body weight ratios in the indicated groups. n = 3 in each group. (H) Histopathological analysis of liver (top row), spleen (middle row), and bone marrow (bottom row) from the indicated chimeras. Tet2⁻/⁻ to WT IgG-treated mice display hepatic myeloid infiltration (dashed outline), splenic white pulp effacement by expanding myeloid elements, and hypercellular bone marrow with increased myeloid-to-erythroid ratio. Scale bars: liver and spleen, 300 μm; bone marrow, 100 μm. All the error bars represent the SEM of the mean. Comparisons among multiple groups were evaluated using a 1-way ANOVA. *p<0.05, **p<0.01. ns: not significant.

Article Snippet: Tet2 floxed mice (#017573), Vav-iCre mice (#008610), and miR-146a knockout mice (#016239) were purchased from the Jackson Laboratory.

Techniques: Blocking Assay, Control, Comparison, Flow Cytometry

(A) Combined UMAP plot of single-cell RNA sequencing data from total bone marrow cells (post erythroid lysis) from Tet2 ⁻/⁻ to WT chimeras treated with IgG or anti-CD40L, compared with Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ chimeras. (B) Individual UMAP plot of the indicated group. The black circle indicates macrophage populations; the red circle indicates T-cell populations. (C) UMAP of macrophage subpopulations across indicated groups. (D) Gene Ontology pathway enrichment analysis of genes enriched in macrophage subpopulation 2. (E) Expression of selected genes in macrophage subpopulation 2. (F) Combined UMAP plot of T-cell subpopulations across all groups. (G) Individual UMAP plot of the indicated group. The T FH -like population is highlighted in a red circle. (H) Expression of indicated genes across T-cell subpopulations in the indicated groups. (I) Representative flow cytometry plots showing CXCR5⁺ICOS⁺CD4⁺ T cells in bone marrow from the indicated groups. (J) Quantification of CXCR5⁺ICOS⁺CD4⁺ T cells from the indicated groups. n = 3 in each group. (K) Adoptive transfer of CD4⁺ T cells from 8-month diseased Tet2 ⁻/⁻ mice or age-matched WT controls into Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ chimeras. Monocyte percentage and hemoglobin levels at one-month post-transfer are presented. All the error bars represent the SEM of the mean. Comparisons among multiple groups were evaluated using a 1-way ANOVA. *p<0.05, **p<0.01, and ***p<0.001. ns: not significant.

Journal: bioRxiv

Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis

doi: 10.64898/2026.05.01.722102

Figure Lengend Snippet: (A) Combined UMAP plot of single-cell RNA sequencing data from total bone marrow cells (post erythroid lysis) from Tet2 ⁻/⁻ to WT chimeras treated with IgG or anti-CD40L, compared with Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ chimeras. (B) Individual UMAP plot of the indicated group. The black circle indicates macrophage populations; the red circle indicates T-cell populations. (C) UMAP of macrophage subpopulations across indicated groups. (D) Gene Ontology pathway enrichment analysis of genes enriched in macrophage subpopulation 2. (E) Expression of selected genes in macrophage subpopulation 2. (F) Combined UMAP plot of T-cell subpopulations across all groups. (G) Individual UMAP plot of the indicated group. The T FH -like population is highlighted in a red circle. (H) Expression of indicated genes across T-cell subpopulations in the indicated groups. (I) Representative flow cytometry plots showing CXCR5⁺ICOS⁺CD4⁺ T cells in bone marrow from the indicated groups. (J) Quantification of CXCR5⁺ICOS⁺CD4⁺ T cells from the indicated groups. n = 3 in each group. (K) Adoptive transfer of CD4⁺ T cells from 8-month diseased Tet2 ⁻/⁻ mice or age-matched WT controls into Tet2 ⁻/⁻ to Gsdmd ⁻/⁻ chimeras. Monocyte percentage and hemoglobin levels at one-month post-transfer are presented. All the error bars represent the SEM of the mean. Comparisons among multiple groups were evaluated using a 1-way ANOVA. *p<0.05, **p<0.01, and ***p<0.001. ns: not significant.

Article Snippet: Tet2 floxed mice (#017573), Vav-iCre mice (#008610), and miR-146a knockout mice (#016239) were purchased from the Jackson Laboratory.

Techniques: Single Cell, RNA Sequencing, Lysis, Expressing, Flow Cytometry, Adoptive Transfer Assay

Journal: bioRxiv

Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis

doi: 10.64898/2026.05.01.722102

Figure Lengend Snippet:

Article Snippet: Tet2 floxed mice (#017573), Vav-iCre mice (#008610), and miR-146a knockout mice (#016239) were purchased from the Jackson Laboratory.

Techniques:

(A) Representative flow cytometry plots showing CD4⁺ and CD8⁺ T-cell populations in bone marrow from patients with isolated TET2 mutations, isolated DNMT3A mutations, and lymphoma-uninvolved reference controls. (B) Quantification of CD4:CD8 T-cell ratios in bone marrow from the indicated patients. n = 6 in lymphoma-uninvolved group; n = 5 in TET2 isolated mutation group; n = 8 in DNMT3A isolated mutation group. The error bars represent the SEM of the mean. The comparison was evaluated using a 1-way ANOVA. ****p<0.0001. ns: not significant. (C) Representative immunohistochemical staining of bone marrow biopsies showing TLS in patients with isolated TET2 mutations. Serial sections were stained for H&E, CD3 (T cells), and CD20 (B cells). TLS was absent in lymphoma-uninvolved reference samples and isolated DNMT3A-mutant cases analyzed. Scale bars: 150 μm. (D) Representative immunofluorescence staining of bone marrow clot sections from the indicated patients. Sections were stained for CD3 (green), CD40L (red), and DAPI (blue). Merged images are shown on the right. Arrows indicate autofluorescence. Scale bars: 10 μm.

Journal: bioRxiv

Article Title: Stromal Gasdermin D-mediated Pyroptosis Drives Maladaptive CD4⁺ T-cell Remodeling in Tet2-Deficient Hematopoiesis

doi: 10.64898/2026.05.01.722102

Figure Lengend Snippet: (A) Representative flow cytometry plots showing CD4⁺ and CD8⁺ T-cell populations in bone marrow from patients with isolated TET2 mutations, isolated DNMT3A mutations, and lymphoma-uninvolved reference controls. (B) Quantification of CD4:CD8 T-cell ratios in bone marrow from the indicated patients. n = 6 in lymphoma-uninvolved group; n = 5 in TET2 isolated mutation group; n = 8 in DNMT3A isolated mutation group. The error bars represent the SEM of the mean. The comparison was evaluated using a 1-way ANOVA. ****p<0.0001. ns: not significant. (C) Representative immunohistochemical staining of bone marrow biopsies showing TLS in patients with isolated TET2 mutations. Serial sections were stained for H&E, CD3 (T cells), and CD20 (B cells). TLS was absent in lymphoma-uninvolved reference samples and isolated DNMT3A-mutant cases analyzed. Scale bars: 150 μm. (D) Representative immunofluorescence staining of bone marrow clot sections from the indicated patients. Sections were stained for CD3 (green), CD40L (red), and DAPI (blue). Merged images are shown on the right. Arrows indicate autofluorescence. Scale bars: 10 μm.

Article Snippet: Tet2 floxed mice (#017573), Vav-iCre mice (#008610), and miR-146a knockout mice (#016239) were purchased from the Jackson Laboratory.

Techniques: Flow Cytometry, Isolation, Mutagenesis, Comparison, Immunohistochemical staining, Staining, Immunofluorescence

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